L'Ifremer
Reconnu dans le monde entier comme l'un des tout premiers instituts en sciences et technologies marines, l'Ifremer s'inscrit dans une double perspective de développement durable et de science ouverte. Il mène des recherches, innove, produit des expertises pour protéger et restaurer l'océan, exploiter ses ressources de manière responsable, et partager les connaissances et les données marines afin de créer de nouvelles opportunités pour une croissance économique respectueuse du milieu marin.
Présents sur toutes les façades maritimes de l'hexagone et des outremers, ses laboratoires sont implantés sur une vingtaine de sites dans les trois grands océans : l'océan Indien, l'Atlantique et le Pacifique. Pour le compte de l'Etat, il opère la Flotte océanographique française au bénéfice de la communauté scientifique nationale. Il conçoit ses propres engins et équipements de pointe pour explorer et observer l'océan, du littoral au grand large et des abysses à l'interface avec l'atmosphère.
Ouverts sur la communauté scientifique internationale, ses 1500 chercheurs, ingénieurs et techniciens font progresser les connaissances sur l'une des dernières frontières inexplorées de notre planète ; ils contribuent à éclairer les politiques publiques et à l'innovation pour une économie bleue durable. Leur mission consiste aussi à sensibiliser le grand public aux enjeux maritimes. Deadline for applications :15/05/2024
General areas of responsibility
EM3B laboratory (fr. Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies) efficiently contributes to the science through fundamental research on marine bacteria from particular environments (food matrices, deep-sea hydrothermal vents) and their valuable metabolites, in particular antimicrobial peptides and exopolysaccharides. This fundamental knowledge opens the way toward innovative developments already explored in food (biopreservation) and medicine (tissue engineering, cancer treatment). https://em3b.ifremer.fr/EM3B-en-bref.
Summary
Three-dimensional (3D) culture models in oncology constitute relevant tools for screening of therapeutic molecules. By reconstructing the complex tumor environment, these in vitro models increase the chances of identifying new drug candidates and thus reduce the number of preclinical animal tests. The 3D model most used in oncology is the culture of cancer cell lines into spheroids or cells from primary cultures into organoids. In order to further mimic the surrounding extracellular environment, these 3D cultures can BE integrated into highly hydrated matrices, made of synthetic or natural polymers, called hydrogels. Through their biological and mechanical properties, hydrogels influence cellular processes, such as viability, migration, proliferation, differentiation and adhesion. Thus, in order to control cellular behavior, IT is essential to dispose artificial extracellular matrices with established functional properties.
In this context, the objective of the thesis is to develop a standardized artificial 3D extracellular matrix with controlled physicochemical and mechanical properties to study the responses of encapsulated cancer cells to new therapeutic approaches. To determine the impact of the established matrix on the viability, morphology, proliferation, differentiation and sensitivity to drugs, cancer cells and cells of their environment (stromal cells, immune cells) formed by lung, pancreas, breast and bone cancers will BE encapsulated in the hydrogels obtained. The matrix allowing the optimal and reproducible development of cellular responses will BE selected for the screening of therapeutic molecules used in oncology (immunotherapy, chemotherapy).
The PhD program is organized into 2 main steps : Step (1) is dedicated to the formulation of hydrogels based on EPS from the Ifremer collection and marine collagen, and their physicochemical and mechanical characterizations. Different parameters (EPS/collagen ratio, concentrations, molecular weight) will BE studied to obtain homogeneous and stable hydrogels in cell culture medium. The impact of the composition of hydrogels on their physicochemical and mechanical properties will then BE determined by Atomic Force Microscopy (AFM) in a physiological environment. Step (2) is devoted to the preparation and encapsulation of cancer cells in the matrices, followed by the characterization of cellular properties in response to various treatments. Cell cultures produced in 3D will make IT possible to screen different therapeutic agents alone or in combination.
Key words
Hydrogel, mechanical properties, cell activity, cancer, therapeutic molecule
En cliquant sur "JE DÉPOSE MON CV", vous acceptez nos CGU et déclarez avoir pris connaissance de la politique de protection des données du site jobijoba.com.