Topic description
Ce projet vise à développer une nouvelle génération de microendoscopes biphotoniques à fibres multi-cœurs, permettant une imagerie rapide, multiplans et volumique couplée à une photostimulation holographique et à l'imagerie de voltage ciblée chez la souris libre de ses mouvements. En intégrant des stratégies haute vitesse compatibles avec l'imagerie de voltage biphotonique et en minimisant les contraintes thermiques, le système ambitionne de dépasser les limitations actuelles en termes de champ de vue et de résolution temporelle. Des expériences seront réalisées dans le cortex visuel et l'hippocampe chez la souris libre de ses mouvements afin de valider notre approche.
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This project aims to develop a new generation of two-photon fiber bundles microendoscopes enabling fast multi-plane and volumetric imaging combined with targeted holographic photostimulation and voltage imaging in freely moving mice. By integrating high-speed strategies compatible with two-photon voltage indicators and optimizing thermal constraints, the system will overcome current limits in field of view and temporal resolution. The approach will be validated through proof-of-principle experiments in the visual cortex and hippocampus during naturalistic behavior.
Aim: The aim of this project is to develop a new generation of 2P-FENDO systems, that enables multi-plane and volumetric operation, maintains high imaging speed over large fields of view, and supports the temporal resolution required for two-photon voltage imaging, while preserving targeted holographic photostimulation in freely moving animals. To achieve this goal, we will investigate and combine multiple complementary strategies to increase imaging speed, expand the accessible imaging and photostimulation volume, and scale the number of simultaneously targeted neurons for voltage imaging. These approaches will be systematically evaluated to identify optimal trade-offs between spatial coverage, temporal resolution, and stimulation flexibility. In parallel, we will adapt a previously developed theoretical framework for temperature rise simulation under two-photon illumination9 to each experimental configuration. For every candidate system design, we will model the expected spatiotemporal thermal distribution in brain tissue, enabling the selection of configurations that maximize endoscope performance while minimizing tissue heating. In collaboration with the neurobiologists of the laboratory, the system will be validated through proof-of-principle experiments in the visual cortex and hippocampus of freely moving mice.
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Début de la thèse : 01/10/
Funding category
Public funding alone (i.e. government, region, European, international organization research grant)
Funding further details
Concours pour un contrat doctoral - SU*
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